|
The original work that was done on pH of the
blood (which is still unfortunately the bench-mark of today's standards)
utilised an isotonic saline (0.160 M NaCl) bridge solution. The
utilisation of this standard is unfortunate because of two main factors:
- This bridge is rarely utilised in advanced scientific research
instrumentation. Tests have shown that a saturated KCl reference
electrode is not only more stable, longer lasting, but has become
the accepted reference cell of choice.
- There is an offset between a 0.160 M NaCl bridge and a
saturated KCl bridge based on the biological fluid being tested. The
offset for whole blood is approximately (-.09 D pH units).
It was therefore a well-documented and educated decision to determine
whether or not to conform to standards being currently accepted, without
compromising the superior function of the KCl bridge. Thereby
mathematically correcting for the variance between the 0.160 M NaCl and
the saturated KCl bridge was considered. However, when the topic was
discussed with numerous research scientists it was determined
unnecessary. Unnecessary as long as documentation was apparent that
would show that there is a recognised variance between theses two
reference cells and their pH readings of biological fluids.
Months of research occurred at both the BTI site in Arizona as
well as an independent laboratory in Houston, Texas. The results
compiled from both facilities correlated with the literature precisely.
The research also determined that saliva is equally effected by the
variance in reference cell composition, but urine is immune to
alterations in this variance.
Ultimately, all of the work that has been done in the field of
Biological Terrain Assessment has revolved around the utilisation of a
KCl reference bridge electrode. BTI does not feel that we can
arbitrarily alter this factor, especially in light of the fact that a
NaCl reference cell will also alter the redox measurement.
Specific research on
this topic came from:
A.H.J. Maas , Clin. Chim. Acta, 28, 373 (1970) J. Appl. Physiol., 30,
248, (1971)
R.N. Khuri and C.R. Merril, Phys. Med. Biol., 9, 541 (1964)
[Top of Page] [BTA Table of Contents]
|